Class 1 Asparaginases

Class Overview

73 experimentally studied proteins

74 sequences in Swiss-Prot

64,081 unique sequences in UniRef100

Bacteria, archaea and eukaryotes (animals, fungi, protozoans, algea)

Affinities to L-asparagine and sometimes L-glutamine or other substrates

N-terminal catalytic and C-terminal stabilizing domains

Tetrameric or dimeric structures

Reference Structure

Clan 1	Clan 2	Clan 3	Clan 4	Clan 5
Family 1 Mostly fungal asparaginases, contains the Saccharomyces cerevisiae isoenzymes.	Family 5 Contains the Bacillus lichenformis asparaginase, a dimeric enzyme with a K_m in the lower millimolar range.	Family 8 A diverse family with poorly conserved motifs.	Family 10 A family of short chain asparaginases that lack the C-terminal stabilizing domain.	Family 14 Mostly animal asparaginases / lysophospholipases with additional ankyrin repeats, like the human and guinea pig enzymes.
Family 2 Putative bacterial asparaginases.	Family 6 Contains the Mycobacterium tuberculosis asparaginase, a dimeric secretory enzyme.	Family 9 Putative bacterial asparaginases.	Family 11 Although a family of mostly bacterial asparaginases, it also contains the dimeric archaeal asparagianses from Pyrococcus furiosus and Thermococcus kodakarensis.	Family 15 Mostly fungal asparaginases with additional ankyrin repeats. Contains the Rhizomucor miehei asparaginase with a Km in the micromolar range.
Family 3 Contains the classic "type II" asparaginases, like the Escherichia coli and Dickeya chrysanthemi asparaginases in pharmaceutical use.	Family 7 Putative bacterial asparaginases with a unique leucine in the conserved Motif 1.		Family 12 The Glutamyl-tRNA amido-transferase subunit D family, a group of achaeal enzymes that also contain the Class 1 asparaginase domain.	Family 16 Asparaginases mostly from Pseudomonadota, most notably the "type I" Escherichia coli and Yersinia pestis enzymes.
Family 4 Putative bacterial asparaginases.			Family 13 Putative bacterial and archaeal asparaginases.	Family 17 A diverse family of putative asparaginases from bacteria and eukaryotes, which often contain an additional unidentified domain.
				Family 18 Almost exclusively putative asparaginases of bacteria belonging to the Bacteroidota phylum.

Experimentally Studied Proteins

Fam ? Class - Clan - Family	Alt ? Alternative historical name / classification	AN ? UniProt accession number	Name ? UniProt entry name, only given here for Swiss-Prot entries	EC	Organism	Cell-Loc	AAs	Structure	PDB	K_m i for Asn [mM]	V_max i for Asn [μmol/min/mg]	K_cat i for Asn [s^-1]
1-1-1	-	i Sequence available in publication. No similar sequences could be found in databases. As presented, lacks the critical conserved Motif 1.		3.5.1.1	Cladosporium sp.	-	370	-	-	100	-	-
1-1-1	-	A0A4D6C6W0		3.5.1.1	Cobetia amphilecti	-	316	-	-	2.05	11641	-
1-1-1	-	C5E3N2		3.5.1.1	Lachancea thermotolerans	-	375	-	-	11.67	-	312.45
1-1-1	ScAII i Identical sequences: P0CZ17, P0CX77, P0CX78, P0CX79	P0CZ17	ASP21_YEAST	3.5.1.1	Saccharomyces cerevisiae	Secreted	362	-	-	0.27	42	-
1-1-1	ScAI	P38986	ASPG1_YEAST	3.5.1.1	Saccharomyces cerevisiae	Cytoplasm	381	-	-	0.075 i K_0.5, allosteric enzyme	-	-
1-1-3	-	P10172	ASPQ_ACIGL	3.5.1.38	Acinetobacter glutaminasificans	Periplasm	331	Homo tetramer	1AGX	-	-	-
1-1-3	-	Q5E4M4		3.5.1.1	Aliivibrio fischeri	-	353	-	-	1.2275	-	-
1-1-3	-	UPI0002D9BB57		3.5.1.1	Bacillus altitudinis	-	382	-	-	0.0147	-	-
1-1-3	-	A0A0P7GBY8		3.5.1.1	Bacillus australimaris	-	382	-	-	0.0456 i Sequence may not be exact match	-	74.6 i Sequence may not be exact match
1-1-3	-	A0A2P8QZG9		3.5.1.1	Campylobacter blaseri	-	347	-	-	1.91	-	13.94
1-1-3	-	Q0PC96		3.5.1.1	Campylobacter jejuni	-	331	Homo tetramer	3NXK	-	-	-
1-1-3	ErA, type II	P06608	ASPG_DICCH	3.5.1.1	Dickeya chrysanthemi	-	348	Homo tetramer	5F52 PDBs	0.058 i Sequence may be not be exact match	-	565 i Sequence may be not be exact match. K_cat may not correspond to K_m.
1-1-3	EcAII	P00805	ASPG2_ECOLI	3.5.1.1	Escherichia coli	Periplasm	348	Homo tetramer	3ECA PDBs	0.015	-	24
1-1-3	-	A0A4Q0YCX3		3.5.1.1	Halarcobacter ebronensis	-	349	-	-	4.27	-	16.55
1-1-3	HpA, type II	Q9ZLB9	ASPG_HELPJ	3.5.1.1	Helicobacter pylori i (strain J99 / ATCC 700824) (Campylobacter pylori J99)	Cytoplasm	332	Homo tetramer	2WLT PDBs	0.29 i S_0.5, Sequence may be not be exact match	31.22 i Sequence may be not be exact match	19.26 i Sequence may be not be exact match
1-1-3	-	UPI0010229EFA		3.5.1.1	Iodobacter sp.	-	355	-	-	1.2	-	82
1-1-3	-	I3DG54		3.5.1.1	Pasteurella bettyae	-	349	-	-	3.98	-	69.12
1-1-3	-	Q7WWK9		3.5.1.1	Pectobacterium atrosepticum i (Erwinia carotovora subsp. atroseptica)	-	349	Homo tetramer	2HLN 90%	0.098	732	1600
1-1-3	-	P10182	ASPQ_PSES7	3.5.1.38	Pseudomonas sp.	Periplasm	337	Homo tetramer	3PGA PDBs	-	-	-
1-1-3	-	i Exact sequence available in publication		3.5.1.38	Pseudomonas sp. i Himalayan Pseudomonas sp. PCH44	-	366	-	-	0.56	-	18.06
1-1-3	-	A0A3R9P3J0		3.5.1.1	Salibacterium salarium i Bacillus salarius	-	359	-	-	5.59	-	8.87
1-1-3	-	A0A0H2WSV5		3.5.1.1	Salmonella paratyphi	-	348	-	-	28	-	39.6
1-1-3	-	A0A240C149		3.5.1.1	Serratia ficaria	-	349	-	-	1.42	-	-
1-1-3	-	A0A1D8KVW6		3.5.1.1	Serratia marcescens i From soil metagenomic libraries generated from forest soil. Possibly Serratia marcescens (estimation).	-	348	-	-	2.0	-	15.5
1-1-3	-	A0A2D3WDS0		3.5.1.1	Sulfurospirillum cavolei	-	348	-	-	0.63	-	10.14
1-1-3	-	D1B4K0		3.5.1.1	Sulfurospirillum deleyianum	-	348	-	-	1.80	-	20.16
1-1-3	WsA, type II	P50286	ASPG_WOLSU	3.5.1.1	Wolinella succinogenes	Cytoplasm	330	Homo tetramer	1WSA PDBs	0.0217	-	97.8
1-1-3	-	D3VBI4		3.5.1.1	Xenorhabdus nematophila	-	347	-	-	4.27	-	6.43
1-1-3	-	Q66CJ2		3.5.1.1	Yersinia pseudotuberculosis	-	345	-	-	0.017	-	-
1-2-5	-	A0A3Q8UES2		3.5.1.1	Bacillus cereus	-	325	Homo dimer i Determined by gel filtration chromatography	-	9.38	-	63.6
1-2-5	BlA, type I	P30363	ASPG_BACLI	3.5.1.1	Bacillus licheniformis	Cytoplasm	322	-	-	-	-	-
1-2-5	i Very similar sequence to ASPG_BACLI, but not identical	A0A6I7U6Y2		3.5.1.1	Bacillus licheniformis	-	322	Homo dimer	7CB4 PDBs	2.96	-	-
1-2-5	i Very similar sequence to ASPG_BACLI, but not identical	A0A3G1GZN5		3.5.1.1	Bacillus licheniformis	-	322	-	-	0.0106	-	23.96
1-2-5	-	Q5KXR8		3.5.1.1	Geobacillus kaustophilus	-	323	-	-	0.487	243.9	-
1-2-5	-	A0A7U9P5Z0		3.5.1.1	Geobacillus thermopakistaniensis	-	323	i "Biochemical characterization revealed that ASNaseGt existed in an oligomeric form in solution which can be converted to the most active tetrameric form by the addition of thiol reducers."	-	0.35	2735	1595
1-2-5	-	F0TGT0		3.5.1.1	Lactobacillus acidophilus	-	331	-	-	6.23	-	0.51
1-2-5	-	A0AAD1ETD3		3.5.1.1	Lactobacillus casei	-	324	-	-	0.0123 i Sequence may not be exact match	-	-
1-2-5	-	F9URL2		3.5.1.1	Lactobacillus plantarum	-	324	-	-	7.85	-	2.33
1-2-5	-	Q38YS9		3.5.1.1	Latilactobacillus sakei	-	324	Homo dimer i Determined by gel filtration	-	7.64	-	4.09
1-2-5	-	I6ZQC6		3.5.1.1	Melioribacter roseus	-	326	-	-	2.7	163	96
1-2-5	i brenda-enzymes.org, archived id J9H7D1	A0A9X4QZ02		3.5.1.1	Staphylococcus sp.	-	324	Homo dimer i Size-exclusion chromatography determined	-	2.2	-	4.65
1-2-5	-	A0A062WR39		3.5.1.1	Streptococcus pneumoniae	-	320	Monomer i Context unknown, paper to be published	9D91 i Paper to be published	-	-	-
1-2-5	-	Q5M2S9		3.5.1.1	Streptococcus thermophilus	-	322	-	-	2.34	-	101
1-2-6	-	A0A1X1VDM8		3.5.1.1	Mycobacterium gordonae	-	312	-	-	6.025	-	194.8
1-2-6	-	A0QX50		3.5.1.1	Mycobacterium smegmatis	-	320	-	-	1.403	-	708.1
1-2-6	-	P9WPX5	ASPG_MYCTU	3.5.1.1	Mycobacterium tuberculosis i (strain ATCC 25618 / H37Rv), identical to P9WPX4	Secreted	315	Dimer	-	0.38 8.3 19.57	74.08 797.7 1587.7	80.7 869.4 1732.5
1-4-10	-	Q2RMX1		3.5.1.1	Rhodospirillum rubrum	-	172	Homo tetramer i Specific tetrameric structure, as this family lacks the C-terminal stabilizing domain present in the other Class 1 families	8UOO PDBs	2.9 3.6 0.280 i Later results indicate a millimolar Km	-	57.1 58.2
1-4-11	-	R4L284 ! Uncertain if sequence and kinetics data match. As presented, the protein lacks the critical conserved Motif 1.		3.5.1.1	Bacillus licheniformis	-	272	-	-	0.671 ! As presented, the protein lacks the critical conserved Motif 1	-	36.8 ! As presented, the protein lacks the critical conserved Motif 1
1-4-11	-	A0A6G7ABJ6		3.5.1.1	Bacillus sonorensis	-	329	-	-	2.004	-	-
1-4-11	-	P26900	ASPG1_BACSU	3.5.1.1	Bacillus subtilis	Cytoplasm	329	-	-	1.579 i Sequence may not be exact match	-	-
1-4-11	-	A0A291B5A4		3.5.1.1	Bacillus tequilensis	-	329	-	-	0.070	-	-
1-4-11	-	A5VMR3		3.5.1.1	Lactobacillus reuteri	-	329	-	-	0.3332 i Sequence may not be exact match	-	-
1-4-11	-	A0A0R2I8I9		3.5.1.1	Limosilactobacillus secaliphilus	-	333	-	-	4.78	-	887
1-4-11	-	Q8TZE8	ASPG_PYRFU	3.5.1.1	Pyrococcus furiosus	-	326	Homo dimer / tetramer i Temperature dependent	5CBP PDBs	12	-	870
1-4-11	PhA	O57797		3.5.1.1	Pyrococcus horikoshii	-	328	Homo dimer	1WLS PDBs	-	-	-
1-4-11	-	F8AHM4		3.5.1.1	Pyrococcus yayanosii	-	328	-	-	6.5	-	-
1-4-11	-	C5A6T2		3.5.1.1	Thermococcus gammatolerans	-	328	-	-	5.3	-	-
1-4-11	-	Q5JIW4	ASPG_THEKO	3.5.1.1	Thermococcus kodakarensis	-	328	Homo dimer	5OT0	2.6 5.5	1121 3300	694
1-4-11	-	C6A532		3.5.1.1	Thermococcus sibiricus	-	331	-	-	4.7	640	400
1-4-11	-	UPI00029ABCE1		3.5.1.1	Thermococcus zilligii	-	330	-	-	6.08	-	3267
1-4-12	-	O26802	GATD_METTH	6.3.5.-	Methanothermobacter thermautotrophicus	-	435	Hetero tetramer i Has additional domain.	2D6F	-	-	-
1-4-12	-	Q9V0T9	GATD_PYRAB	6.3.5.-	Pyrococcus abyssi	-	438	Hetero tetramer i Has additional domain.	1ZQ1	-	-	-
1-5-14	CpAI	H0W0T5		3.5.1.1	Cavia porcellus	-	565	Homo tetramer i Additional ankrytin repeats.	4R8L PDBs	0.057	-	38.6
1-5-14	hAI	Q86U10	LPP60_HUMAN	3.1.1.5 3.5.1.1 3.1.1.47	Homo sapiens	-	573	Monomer i At assay conditions, substrate-inducing effect on the oligomeric state	-	11.5 i S_0.5, alloseric enzyme	-	6.7
1-5-15	RmAI	W0G253		3.5.1.1	Rhizomucor miehei	-	682	Homo dimer i Determined by gel filtration. Has additional ankyrin repeats.	-	0.133 i brenda-enzymes.org	3380.0	676
1-5-16	EcAI i Identical sequences: P0A963, P0A962	P0A962	ASPG1_ECOLI	3.5.1.1	Escherichia coli i (strain K12)	Cytoplasm	338	Homo tetramer i dimer of two intimate dimers	2HIM PDBs	1.2 i S_0.5, allosteric enzyme 3.5	-	-
1-5-16	-	A0A221KKE2		3.5.1.1	Halomonas elongata	-	355	-	-	5.6	-	1960
1-5-16	-	X0NLX5		3.5.1.1	Photobacterium leiognathi i Fish gut microbiome. Organism not certain.	-	337	-	-	3.008 i Sequence may not be exact match	-	-
1-5-16	i As presented, lacks the critical conserved Motif 1	A0A7D7L7P6		3.5.1.1	Pseudomonas aeruginosa	-	306	-	-	10.904	-	-
1-5-16	-	A0A2D5S0K3		3.5.1.1	Rheinheimera sp	-	341	-	-	0.98	-	16.17
1-5-16	VcA, type I	Q9KQK3		3.5.1.1	Vibrio cholerae	Cytoplasm	337	Homo tetramer	2OCD i Paper to be published	1.1 i Sequence may not be exact match	-	4424 i Sequence may not be exact match
1-5-16	-	A0A191W2U6		3.5.1.1	Vibrio sp.	-	337	-	-	4.517	-	2.88
1-5-16	YpAI	A0A3N4B0Q2		3.5.1.1	Yersinia pestis	Cytoplasm	338	Homo dimer / tetramer	7R69 PDBs	6.0 i K_0.5	-	42.0

Swiss-Prot Sequences

Fam ? Class - Clan - Family	Alt ? Alternative historical name / classification	AN ? UniProt accession number	Name ? UniProt entry name, only given here for Swiss-Prot entries	EC	Organism	Cell-Loc	AAs	Structure	PDB	K_m i for Asn [mM]	V_max i for Asn [μmol/min/mg]	K_cat i for Asn [s^-1]
1-1-1	ScAII i Identical sequences: P0CZ17, P0CX77, P0CX78, P0CX79	P0CZ17	ASP21_YEAST	3.5.1.1	Saccharomyces cerevisiae	Secreted	362	-	-	0.27	42	-
1-1-1	ScAII i Identical sequences: P0CZ17, P0CX77, P0CX78, P0CX79	P0CX77	ASP22_YEAST	3.5.1.1	Saccharomyces cerevisiae	Secreted	362	-	-	0.27	42	-
1-1-1	ScAII i Identical sequences: P0CZ17, P0CX77, P0CX78, P0CX79	P0CX78	ASP23_YEAST	3.5.1.1	Saccharomyces cerevisiae	Secreted	362	-	-	0.27	42	-
1-1-1	ScAII i Identical sequences: P0CZ17, P0CX77, P0CX78, P0CX79	P0CX79	ASP24_YEAST	3.5.1.1	Saccharomyces cerevisiae	Secreted	362	-	-	0.27	42	-
1-1-1	-	P87015	ASPG1_SCHPO	3.5.1.1	Schizosaccharomyces pombe	Secreted, cell wall	360	-	-	-	-	-
1-1-1	ScAI	P38986	ASPG1_YEAST	3.5.1.1	Saccharomyces cerevisiae	Cytoplasm	381	-	-	0.075 i K_0.5, allosteric enzyme	-	-
1-1-1	-	Q9UTS7	ASPG2_SCHPO	3.5.1.1	Schizosaccharomyces pombe	Secreted, cell wall	356	-	-	-	-	-
1-1-1	-	Q8NKC0	ASPG3_SCHPO	3.5.1.1	Schizosaccharomyces pombe	Secreted, cell wall	360	-	-	-	-	-
1-1-1	-	Q8TFF8	ASPG4_SCHPO	3.5.1.1	Schizosaccharomyces pombe	Secreted, cell wall	356	-	-	-	-	-
1-1-3	-	O34482	ASPG2_BACSU	3.5.1.1	Bacillus subtilis	-	375	-	-	0.43	-	-
1-1-3	EcAII	P00805	ASPG2_ECOLI	3.5.1.1	Escherichia coli	Periplasm	348	Homo tetramer	3ECA PDBs	0.015	-	24
1-1-3	-	P43843	ASPG2_HAEIN	3.5.1.1	Haemophilus influenzae	Periplasm	349	-	-	-	-	-
1-1-3	ErA, type II	P06608	ASPG_DICCH	3.5.1.1	Dickeya chrysanthemi	-	348	Homo tetramer	5F52 PDBs	0.058 i Sequence may be not be exact match.	-	565 i Sequence may be not be exact match. K_cat may not correspond to K_m.
1-1-3	HpA, type II	Q9ZLB9	ASPG_HELPJ	3.5.1.1	Helicobacter pylori i (strain J99 / ATCC 700824) (Campylobacter pylori J99)	Cytoplasm	332	Homo tetramer	2WLT PDBs	0.29 i S_0.5, Sequence may be not be exact match	31.22 i Sequence may be not be exact match	19.26 i Sequence may be not be exact match
1-1-3	HpA, type II	O25424	ASPG_HELPY	3.5.1.1	Helicobacter pylori i (strain ATCC 700392 / 26695) (Campylobacter pylori)	Cytoplasm	330	-	-	-	-	-
1-1-3	WsA, type II	P50286	ASPG_WOLSU	3.5.1.1	Wolinella succinogenes	Cytoplasm	330	Homo tetramer	1WSA PDBs	0.0217	-	97.8
1-1-3	-	P10172	ASPQ_ACIGL	3.5.1.38	Acinetobacter glutaminasificans	Periplasm	331	Homo tetramer	1AGX	-	-	-
1-1-3	-	Q9I407	ASPQ_PSEAE	3.5.1.38	Pseudomonas aeruginosa	Periplasm	362	-	-	-	-	-
1-1-3	-	O68897	ASPQ_PSEFA	3.5.1.38	Pseudomonas fluorescens	Periplasm	362	-	-	-	-	-
1-1-3	-	Q88K39	ASPQ_PSEPK	3.5.1.38	Pseudomonas putida	Periplasm	362	-	-	-	-	-
1-1-3	-	P10182	ASPQ_PSES7	3.5.1.38	Pseudomonas sp.	Periplasm	337	Homo tetramer	3PGA PDBs	-	-	-
1-2-5	BlA, type I	P30363	ASPG_BACLI	3.5.1.1	Bacillus licheniformis	Cytoplasm	322	-	-	-	-	-
1-2-6	-	Q9RRX9	ASPG_DEIRA	3.5.1.1	Deinococcus radiodurans	Cytoplasm	322	-	-	-	-	-
1-2-6	-	P63628	ASPG_MYCBO	3.5.1.1	Mycobacterium bovis	Secreted	315	-	-	-	-	-
1-2-6	-	Q9X7E6	ASPG_MYCLE	3.5.1.1	Mycobacterium leprae	Secreted	310	-	-	-	-	-
1-2-6	-	P9WPX4	ASPG_MYCTO	3.5.1.1	Mycobacterium tuberculosis i (strain CDC 1551 / Oshkosh), identical to P9WPX5	Secreted	315	-	-	-	-	-
1-2-6	-	P9WPX5	ASPG_MYCTU	3.5.1.1	Mycobacterium tuberculosis i (strain ATCC 25618 / H37Rv), identical to P9WPX4	Secreted	315	Dimer	-	0.38 8.3 19.57	74.08 797.7 1587.7	80.7 869.4 1732.5
1-4-11	-	P26900	ASPG1_BACSU	3.5.1.1	Bacillus subtilis	Cytoplasm	329	-	-	1.579 i Sequence may not be exact match	-	-
1-4-11	-	Q8TZE8	ASPG_PYRFU	3.5.1.1	Pyrococcus furiosus	-	326	Homo dimer / tetramer i Temperature dependent	5CBP PDBs	12	-	870
1-4-11	-	Q5JIW4	ASPG_THEKO	3.5.1.1	Thermococcus kodakarensis	-	328	Homo dimer	5OT0	2.6 5.5	1121 3300	694
1-4-12	-	Q9Y9T8	GATD_AERPE	6.3.5.-	Aeropyrum pernix	-	427	-	-	-	-	-
1-4-12	-	O29380	GATD_ARCFU	6.3.5.-	Archaeoglobus fulgidus	-	418	-	-	-	-	-
1-4-12	-	B0R6H4	GATD_HALS3	6.3.5.-	Halobacterium salinarum	-	427	-	-	-	-	-
1-4-12	-	Q9HP20	GATD_HALSA	6.3.5.-	Halobacterium salinarum	-	427	-	-	-	-	-
1-4-12	-	Q18GL3	GATD_HALWD	6.3.5.-	Haloquadratum walsbyi	-	442	-	-	-	-	-
1-4-12	-	Q8TR66	GATD_METAC	6.3.5.-	Methanosarcina acetivorans	-	424	-	-	-	-	-
1-4-12	-	Q46GJ6	GATD_METBF	6.3.5.-	Methanosarcina barkeri	-	424	-	-	-	-	-
1-4-12	-	Q12X65	GATD_METBU	6.3.5.-	Methanococcoides burtonii	-	415	-	-	-	-	-
1-4-12	-	Q60331	GATD_METJA	6.3.5.-	Methanocaldococcus jannaschii	-	417	-	-	-	-	-
1-4-12	-	Q8TV84	GATD_METKA	6.3.5.-	Methanopyrus kandleri	-	458	-	-	-	-	-
1-4-12	-	A4FWR5	GATD_METM5	6.3.5.-	Methanococcus maripaludis	-	418	-	-	-	-	-
1-4-12	-	A9AA46	GATD_METM6	6.3.5.-	Methanococcus maripaludis	-	418	-	-	-	-	-
1-4-12	-	A6VGK5	GATD_METM7	6.3.5.-	Methanococcus maripaludis	-	418	-	-	-	-	-
1-4-12	-	Q8PUM7	GATD_METMA	6.3.5.-	Methanosarcina mazei	-	425	-	-	-	-	-
1-4-12	-	P61400	GATD_METMP	6.3.5.-	Methanococcus maripaludis	-	418	-	-	-	-	-
1-4-12	-	A5UK11	GATD_METS3	6.3.5.-	Methanobrevibacter smithii	-	436	-	-	-	-	-
1-4-12	-	A4YHH3	GATD_METS5	6.3.5.-	Metallosphaera sedula	-	439	-	-	-	-	-
1-4-12	-	Q2NEH1	GATD_METST	6.3.5.-	Methanosphaera stadtmanae	-	438	-	-	-	-	-
1-4-12	-	O26802	GATD_METTH	6.3.5.-	Methanothermobacter thermautotrophicus	-	435	Hetero tetramer i Has additional domain.	2D6F	-	-	-
1-4-12	-	A6UPR4	GATD_METVS	6.3.5.-	Methanococcus vannielii	-	426	-	-	-	-	-
1-4-12	-	P61401	GATD_NANEQ	6.3.5.-	Nanoarchaeum equitans	-	392	-	-	-	-	-
1-4-12	-	Q9V0T9	GATD_PYRAB	6.3.5.-	Pyrococcus abyssi	-	438	Hetero tetramer i Has additional domain.	1ZQ1	-	-	-
1-4-12	-	Q8ZY04	GATD_PYRAE	6.3.5.-	Pyrobaculum aerophilum	-	417	-	-	-	-	-
1-4-12	-	Q8U0X0	GATD_PYRFU	6.3.5.-	Pyrococcus furiosus	-	438	-	-	-	-	-
1-4-12	-	O59132	GATD_PYRHO	6.3.5.-	Pyrococcus horikoshii	-	438	-	-	-	-	-
1-4-12	-	Q97ZH5	GATD_SACS2	6.3.5.-	Saccharolobus solfataricus	-	444	-	-	-	-	-
1-4-12	-	Q4J955	GATD_SULAC	6.3.5.-	Sulfolobus acidocaldarius	-	446	-	-	-	-	-
1-4-12	-	C3N5E7	GATD_SULIA	6.3.5.-	Sulfolobus islandicus	-	445	-	-	-	-	-
1-4-12	-	C4KH13	GATD_SULIK	6.3.5.-	Sulfolobus islandicus	-	445	-	-	-	-	-
1-4-12	-	C3MPS1	GATD_SULIL	6.3.5.-	Sulfolobus islandicus	-	445	-	-	-	-	-
1-4-12	-	C3MYR5	GATD_SULIM	6.3.5.-	Sulfolobus islandicus	-	445	-	-	-	-	-
1-4-12	-	C3NHQ2	GATD_SULIN	6.3.5.-	Sulfolobus islandicus	-	445	-	-	-	-	-
1-4-12	-	C3NE01	GATD_SULIY	6.3.5.-	Sulfolobus islandicus	-	445	-	-	-	-	-
1-4-12	-	Q971W5	GATD_SULTO	6.3.5.-	Sulfurisphaera tokodaii	-	448	-	-	-	-	-
1-4-12	-	Q9HJJ5	GATD_THEAC	6.3.5.-	Thermoplasma acidophilum	-	409	-	-	-	-	-
1-4-12	-	Q5JI77	GATD_THEKO	6.3.5.-	Thermococcus kodakarensis	-	440	-	-	-	-	-
1-4-12	-	A1RX40	GATD_THEPD	6.3.5.-	Thermofilum pendens	-	451	-	-	-	-	-
1-4-12	-	Q979L8	GATD_THEVO	6.3.5.-	Thermoplasma volcanium	-	406	-	-	-	-	-
1-5-14	-	Q9U518	ASPG_DIRIM	3.5.1.1	Dirofilaria immitis	-	590	-	-	-	-	-
1-5-14	hAI	Q86U10	LPP60_HUMAN	3.1.1.5 3.5.1.1 3.1.1.47	Homo sapiens	-	573	Monomer i At assay conditions, substrate-inducing effect on the oligomeric state. Has additional ankyrin repeats.	-	11.5 i S_0.5, alloseric enzyme	-	6.7
1-5-14	-	A0JNU3	LPP60_MOUSE	3.1.1.5 3.5.1.1 3.1.1.47	Mus musculus	-	564	-	-	-	-	-
1-5-14	-	O88202	LPP60_RAT	3.1.1.5 3.5.1.1 3.1.1.47	Rattus norvegicus	-	564	-	-	-	-	-
1-5-16	EcAI i Identical sequences: P0A963, P0A962	P0A963	ASPG1_ECO57	3.5.1.1	Escherichia coli i (O157:H7)	Cytoplasm	338	-	-	-	-	-
1-5-16	EcAI i Identical sequences: P0A963, P0A962	P0A962	ASPG1_ECOLI	3.5.1.1	Escherichia coli i (strain K12)	Cytoplasm	338	Homo tetramer i dimer of two intimate dimers	2HIM PDBs	1.2 i S_0.5, allosteric enzyme 3.5	-	-

UniRef100 Sequences

Phylogenetic Tree and Introduction

Class 1 L-asparaginases, previously bacterial-type L-asparaginases, are the largest and most studied class that contain both L-asparaginases in pharmaceutical use. Class 1 L-asparaginases can be found in bacteria, archaea, and eukaryotes, including humans. They display a range of affinities to L-asparagine, L-glutamine and sometimes other substrates, such as 1-acyl-sn-glycero-3-phosphocholine or L-glutamyl-tRNA(Gln), however the L-asparaginases with the highest affinities to L-asparagine can be found in this class.

Although sequence similarity can be quite low between sequences, the tertiary structure of the L-sparaginase domain is well conserved throughout the class. This class typically consists of a N-terminal catalytic domain and a C-terminal stabilizing domain connected by a linker, which make up the Class 1 L-asparaginase monomer. An exception to this are Family 10 L-asparaginases, which lack the C-terminal stabilizing domain. Family 14 and Family 15 also tend to have additional ankyrin repeats.

Structurally, the L-asparaginases in this class tend to organize into tetramers and sometimes dimers.

Phylogenetic tree

Representative Sequence Alignment

Conserved Motifs of Families

Families Overview

Clan	Family	UniProt ID: (name, PDB)	Activity	Extra domains	Protein structure	Molecular weight	Taxonomy	Cell location	K_m (Asn)
1	1	S: P38986 (ScAI) S: P0CX78 (ScAII)	ASNase with minimal GLNase	signal seq. (most)	-	~40 kDa	eukaryotes (fungi) bacteria (some)	secreted cytoplasm	μM mM
	2	U: A0A931LDC7 U: E3QQI5	ASNase (putative)	signal seq. (some)	-	-	bacteria	-	-
	3	S: P00805 (EcAII, 3ECA) S: P06608 (ErAII, 5F52)	ASNase with low GLNase GLNase with lower ASNase	signal seq. (some)	homo tetramer	~35 kDa	bacteria	periplasm cytoplasm	μM mM
	4	U: W7NWT6 U: A0A0N7J905	ASNase (putative)	signal seq. (few)	-	-	bacteria	-	-
2	5	S*: P30363 (BlA, 7C8X i Sequence may be not be exact match ) U: U5T4E5	ASNase with minimal GLNase	-	homo dimer	~37 kDa	bacteria	-	μM mM
	6	S*: P9WPX5 (MtA) S: Q9RRX9	ASNase	-	dimer	~36 kDa	bacteria	secreted	mM
	7	U: C1B6A9 U: U2BE30	ASNase (putative)	-	-	-	bacteria	-	-
3	8	U: E3CWW1 U: K0KG84	ASNase (putative)	disorder. regions (some) signal seq. (some) repeats (some)	-	-	bacteria eukaryotes (algae, fungi, metazoans)	-	-
3	9	U: A0A6N3EMU0 U: A0A928JPL4	ASNase (putative)	-	-	-	bacteria	-	-
4	10	U*: Q2RMX1 (RrA, 8UOO) U: G2IJ92	ASNase	lack C-terminal ASNase domain	homo tetramer	~18 kDa	bacteria	-	mM
	11	S: Q8TZE8 (PfA, 4Q0M) S: Q5JIW4 (TkA, 5OT0)	ASNase	-	homo dimer	~37 kDa	bacteria (mostly bacillota) archaea	cytoplasm	μM mM
	12	S: Q9V0T9 (1ZQ1) S: O26802 (2D6F)	glutamyl-tRNA amido-transferase subunit D	extra ~100 amino acid domain	hetero tetramer	~50 kDa	archaea bacteria (some)	-
	13	U: K2BPI2 U: B7G6G9	ASNase (putative)	-	-	-	bacteria archaea (some)	-	-
5	14	S: Q86U10 (HsAI) U: H0W0T5 (CpAI, 4R8L)	lysophospholipase, trans-acetylase, acetyl-hydrolase, ASNase	ankyrin repeats (most)	tetramer (dimer of dimers)	~60 kDa	eukaryotes (mostly metazoans)	-	μM mM
	15	U*: W0G253 (RmAI) U: D0LXX2	ASNase with minimal GLNase	ankyrin repeats (most)	homo dimer (putative)	~72 kDa	eukaryotes (mostly fungi) bacteria (some)	-	μM
	16	S: P0A962 (EcAI, 2HIM) U: A0A3N4B0Q2 (YpAI, 7R69)	ASNase with minimal GLNase	-	tetramer (dimer of two intimate dimers) homo dimer	~37 kDa	bacteria (mostly pseudo-monadota)	cytoplasm	mM
	17	U: A0A318KGZ9 U: A0A1G7HJG0	ASNase (putative)	additional amino acid chain (often) C-terminal domain (often)	-	-	bacteria eukaryotes (sar, algae, fungi, discoba)	-	-
	18	U: A0A653PC67 U: G8R309	ASNase (putative)	-	-	-	bacteria (bacteroidota)	-	-

Entry: S = Swiss-Prot, U = UniProtKB, P = UniParc, * = experimentally studied protein, UniProt accession number (short protein name, PDB id). Activity: ASNase = L-asparaginase, GLNase = L-glutaminase. Molecular weight is for a monomeric unit. Km is for L-asparagine: μM = Km < 1 mM, mM = Km ≥ 1 mM.