19 experimentally studied proteins
3 sequences in Swiss-Prot
3,149 unique sequences in UniRef100
Bacterial and archaeal L-asparaginases
High thermal stability and activity
Dimeric asparagianses from Pyrococcus furiosus and Thermococcus kodakarensis
| Fam ? Class - Clan - Family | Alt ? Alternative historical name / classification | AN ? UniProt accession number | Name ? UniProt entry name, only given here for Swiss-Prot entries | EC | Organism | Cell-Loc | AAs | Structure | PDB | Km i for Asn [mM] | Vmax i for Asn [μmol/min/mg] | Kcat i for Asn [s-1] | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1-4-11 | - | R4L284 ! Uncertain if sequence and kinetics data match. As presented, the protein lacks the critical conserved Motif 1. | 3.5.1.1 | Bacillus licheniformis | - | 272 | - | - | 0.671 ! As presented, the protein lacks the critical conserved Motif 1 | - | 36.8 ! As presented, the protein lacks the critical conserved Motif 1 | ||
| 1-4-11 | - | A0A6G7ABJ6 | 3.5.1.1 | Bacillus sonorensis | - | 329 | - | - | 2.004 | - | - | ||
| 1-4-11 | - | P26900 | ASPG1_BACSU | 3.5.1.1 | Bacillus subtilis | Cytoplasm | 329 | - | - | 1.579 i Sequence may not be exact match | - | - | |
| 1-4-11 | - | A0A291B5A4 | 3.5.1.1 | Bacillus tequilensis | - | 329 | - | - | 0.070 | - | - | ||
| 1-4-11 | - | A5VMR3 | 3.5.1.1 | Lactobacillus reuteri | - | 329 | - | - | 0.3332 i Sequence may not be exact match | - | - | ||
| 1-4-11 | - | A0A0R2I8I9 | 3.5.1.1 | Limosilactobacillus secaliphilus | - | 333 | - | - | 4.78 | - | 887 | ||
| 1-4-11 | - | Q8TZE8 | ASPG_PYRFU | 3.5.1.1 | Pyrococcus furiosus | - | 326 | Homo dimer / tetramer i Temperature dependent | 5CBP PDBs | 12 | - | 870 | |
| 1-4-11 | PhA | O57797 | 3.5.1.1 | Pyrococcus horikoshii | - | 328 | Homo dimer | 1WLS PDBs | - | - | - | ||
| 1-4-11 | - | F8AHM4 | 3.5.1.1 | Pyrococcus yayanosii | - | 328 | - | - | 6.5 | - | - | ||
| 1-4-11 | - | C5A6T2 | 3.5.1.1 | Thermococcus gammatolerans | - | 328 | - | - | 5.3 | - | - | ||
| 1-4-11 | - | Q5JIW4 | ASPG_THEKO | 3.5.1.1 | Thermococcus kodakarensis | - | 328 | Homo dimer | 5OT0 |
2.6 5.5 |
1121 3300 | 694 | |
| 1-4-11 | - | C6A532 | 3.5.1.1 | Thermococcus sibiricus | - | 331 | - | - | 4.7 | 640 | 400 | ||
| 1-4-11 | - | UPI00029ABCE1 | 3.5.1.1 | Thermococcus zilligii | - | 330 | - | - | 6.08 | - | 3267 |
| Fam ? Class - Clan - Family | Alt ? Alternative historical name / classification | AN ? UniProt accession number | Name ? UniProt entry name, only given here for Swiss-Prot entries | EC | Organism | Cell-Loc | AAs | Structure | PDB | Km i for Asn [mM] | Vmax i for Asn [μmol/min/mg] | Kcat i for Asn [s-1] | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1-4-11 | - | P26900 | ASPG1_BACSU | 3.5.1.1 | Bacillus subtilis | Cytoplasm | 329 | - | - | 1.579 i Sequence may not be exact match | - | - | |
| 1-4-11 | - | Q8TZE8 | ASPG_PYRFU | 3.5.1.1 | Pyrococcus furiosus | - | 326 | Homo dimer / tetramer i Temperature dependent | 5CBP PDBs | 12 | - | 870 | |
| 1-4-11 | - | Q5JIW4 | ASPG_THEKO | 3.5.1.1 | Thermococcus kodakarensis | - | 328 | Homo dimer | 5OT0 |
2.6 5.5 |
1121 3300 | 694 |
Clan 4 is an interesting clan of bacterial and archaeal L-asparaginases. Some sequences display characteristics of both "type I" and "type II" L-asparaginases and some are structurally unique.
Family 11 L-asparaginases are mostly bacterial, although there are important archaeal representatives present. Three protein structures of L-asparaginases have been solved in this family, all proteins of archaeal origin: Pyrococcus furiosus PfA (Q8TZE8), Pyrococcus horikoshii PhA (O57797) and the cytoplasmic Thermococcus kodakarensis TkA (Q5JIW4). These L-asparaginases have a homo dimeric native form and the Km for L-asparagine tends to be in the millimolar range. These particular L-asparaginases have likely been chosen for structure analysis due to their archaeal hyperthermophilic origin, but they show high structural similarity (~0.95 TM-score) to numerous bacterial L-asparaginases in the same family, such as the Bacillus subtilis BsA (P26900).
